GEO series
DNA damage hotspots for the Top2i "ICRF193"
GSE256090
Mus musculus
Genome binding/occupancy profiling by high throughput sequencing
12 samples
2024/04/17
GPL21103
Summary
In our project we are using the Top2 inhibitor, ICRF-193, in order to induce damage in NIH3T3 MEFs. We report the high-throughput identification of genomic hotspots for damage induction by ICRF-193, using γH2AX as a marker. We find that ICRF-193 primarily induces damage in repetitive sequences, which are known to reside in regions of heterochromatin, with a very small portion of damaged sites residing in regions of active genes in euchromatin. Overall, this study provides evidence that catalytic inhibition of Top2 enzymes by ICRF-193, leads to induction of damage in the nuclear sub-compartments of heterochromatin.
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Paper (PMID 38977669) ↗
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