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Effect of ECP on intestinal immune cells in immune checkpoint inhibitor-induced colitis - batch2 [scRNA-Seq]

GSE256286 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2024/11/27 Platform GPL24247
Summary
Immune Checkpoint Inhibitors (ICI) have proven to be beneficial against tumors by boosting anti-tumor immunity, however these therapies can induce severe inflammatory side effects in all tissues, named immune-related adverse events (irAE). The colon is affected most commonly. These cause termination of treatment. Until today, patients with irAEs received systemic immunosuppressive therapies. We have found ECP as a novel therapy, which causes immunomodulation in the inflamed tissue without systemic immunosuppression. To understand which immune cells are affected by ECP in the inflamed intestinal tract, mice were treated with DSS and anti-PD1 to induce colitis, comparable to patients suffering from ICI-induced irAEs. ECP showed beneficial effects in patients. Mice were transplanted with ECP-treated cells as treatment and scSeq was performed with Colon infiltrating CD45 positive cells. Naive mice, mice receiving DSS and isotype control and mice receiving DSS in combination with anti-PD1 and untreated splenocytes, served as control.
Published in
Adiponectin reduces immune checkpoint inhibitor-induced inflammation without blocking anti-tumor immunity
Braun LM, Giesler S, Andrieux G et al. · Cancer cell 2025 · PMID 39933899 · doi:10.1016/j.ccell.2025.01.004
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Direct links to NCBI, no account and no request form: the whole study as GSE256286_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1078864 and SRA study SRP490862. Searching any of these in the dataset finder brings you back here.

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