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Semaglutide-induced Loss of Skeletal Muscle Mass is Blunted by Co-administration of Ketone Esters

GSE326952 Mus musculus (mouse) RNA-seq (gene expression) 13 samples Submitted 2026/05/27 Platform GPL30172
At a glance

RNA-seq (gene expression) · mouse · 13 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
13 samples: 13 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Building on our recent work demonstrating that ketone ester therapy prevents semaglutide-induced cardiac mass loss in obese mice, we investigated whether ketone therapy could also preserve skeletal muscle mass during semaglutide treatment. To characterize the molecular changes associated with this treatment, we performed RNA-seq transcriptomic analysis of gastrocnemius muscle from obese mice treated with vehicle, semaglutide, or semaglutide + ketone ester.
Read the full summary ↓
Our analysis identified 5 dominant transcriptomic clusters enriched for pathways related to mitochondrial function, muscle contraction, translation, proteostasis, and extracellular matrix organization. Semaglutide increased expression of genes related to proteostasis and translation, whereas these changes were normalized with ketone ester co-treatment. In addition, semaglutide suppressed several mitochondrial genes, which were restored with ketone ester co-treatment. Furthermore, semaglutide reduced the expression of genes involved in the mitochondrial electron transport chain while increasing the expression of atrophy related genes, both of which were reversed by ketone ester co-treatment. Collectively, these findings suggest that ketone ester co-therapy mitigates semaglutide-induced transcriptomic alterations in skeletal muscle, providing mechanistic insight into how ketone therapy may help preserve muscle function during GLP-1based weight-loss therapy.
This dataset
Open GSE326952 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE326952_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1447990 and SRA study SRP689148. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
13 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 13 sample titles: 13 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.