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Arginine methylation-mediated FXR1 binding with RNAs in cancer cells

GSE256352 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2024/04/08 Platform GPL24676
Summary
FXR1 is an RNA binding protein and it is post traslationally modified by a methyltrasferase to facilitate its RNA binding activity FXR1 is methylated by PRMT5, an arginine methyltransferase, to promote its stability and facilitate its association with mRNAs. Both genetic and small molecule PRMT5 inhibition failed to methylate recombinant as well as the endogenous FXR1, resulting in protein instability and downregulation of FXR1 target mRNA levels in HNSCC cells. To study the differentially regulated gene under the loss of PRMT5, we knocked- down the PRMT5 using shRNA and confirmed the transcript and protein levels using qRT PCR and immunoblot respectively.
Published in
PRMT5-mediated arginine methylation of FXR1 is essential for RNA binding in cancer cells
Vijayakumar A, Majumder M, Yin S et al. · Nucleic acids research 2024 · PMID 38709899 · doi:10.1093/nar/gkae319
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Also filed as BioProject PRJNA1079254 and SRA study SRP491009. Searching any of these in the dataset finder brings you back here.

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