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The impact of inhibiting Dgat2 and Pnpla2 functional enhancers on the transcriptome of 3T3-L1 adipocytes.

GSE259270 Mus musculus Expression profiling by high throughput sequencing 9 samples Submitted 2025/02/05 Platform GPL24247
Summary
In order to explore the impact of enhancer inhibition on adipocyte differentiation and triglyceride deposition at the transcriptome level, we infected cells with lentiviral vectors containing dCas9-KRAB-sgRNAs (experimental group) or dCas9-KRAB-Control (control group) at a multiplicity of infection (MOI) of 100 for 12 hours in 1 mL culture medium. Transcriptome analysis via RNA sequencing (RNA-seq) was conducted on dCas9-KRAB-Dgat2-En4, dCas9-KRAB-Pnpla2-En5, and dCas9-KRAB-Control cells at day 7 of differentiation.
Published in
Inhibition of triglyceride metabolism-associated enhancers alters lipid deposition during adipocyte differentiation
Zeng S, Li Z, Li X et al. · FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2025 · PMID 39873971 · doi:10.1096/fj.202401137R
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Also filed as BioProject PRJNA1080560 and SRA study SRP491789. Searching any of these in the dataset finder brings you back here.

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