In vitro modeling of nutritional and mitochondrial-targeted therapies for osteosarcoma
RNA-seq (gene expression) · human · 98 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Topic
- OsteosarcomaThis series is part of our curated briefing on it.
- Samples
- 98 samples: 33 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- In vitro modeling of nutritional and mitochondria-targeted therapies for osteosarcomabioRxiv : the preprint server for biology · 2026
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE259286_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1080588 and SRA study SRP491863. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 97 samples.
How we read this: 33 replicated groups read from 98 sample titles; they account for 97 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8113089 143B, ONC201, Replicate 1 143B, ONC201,
- GSM8113090 143B, ONC201, Replicate 2 143B, ONC201,
- GSM8113091 143B, ONC201, Replicate 3 143B, ONC201,
- GSM8113092 143B, ONC206, Replicate 1 143B, ONC206,
- GSM8113093 143B, ONC206, Replicate 2 143B, ONC206,
- GSM8113094 143B, ONC206, Replicate 3 143B, ONC206,
- GSM8113095 143B, cycloheximide, Replicate 1 143B, cycloheximide,
- GSM8113096 143B, cycloheximide, Replicate 2 143B, cycloheximide,
- GSM8113097 143B, cycloheximide, Replicate 3 143B, cycloheximide,
- GSM8113098 143B, galactose, Replicate 1 143B, galactose,
- GSM8113099 143B, galactose, Replicate 2 143B, galactose,
- GSM8113100 143B, galactose, Replicate 3 143B, galactose,
- GSM8113101 143B, glucose, Replicate 1 143B, glucose,
- GSM8113102 143B, glucose, Replicate 2 143B, glucose,
- GSM8113103 143B, glucose, Replicate 3 143B, glucose,
- GSM8113104 143B, metformin, Replicate 1 143B, metformin,
- GSM8113105 143B, metformin, Replicate 2 143B, metformin,
- GSM8113106 143B, metformin, Replicate 3 143B, metformin,
- GSM8113107 15454, ONC201, Replicate 1 15454, ONC201,
- GSM8113108 15454, ONC201, Replicate 2 15454, ONC201,
- GSM8113109 15454, ONC201, Replicate 3 15454, ONC201,
- GSM8113110 15454, ONC206, Replicate 1 15454, ONC206,
- GSM8113111 15454, ONC206, Replicate 2 15454, ONC206,
- GSM8113112 15454, ONC206, Replicate 3 15454, ONC206,
- GSM8113113 15454, cycloheximide, Replicate 1 15454, cycloheximide,
- GSM8113114 15454, cycloheximide, Replicate 2 15454, cycloheximide,
- GSM8113115 15454, cycloheximide, Replicate 3 15454, cycloheximide,
- GSM8113116 15454, galactose, Replicate 1 15454, galactose,
- GSM8113117 15454, galactose, Replicate 2 15454, galactose,
- GSM8113118 15454, galactose, Replicate 3 15454, galactose,
- GSM8113119 15454, glucose, Replicate 1 15454, glucose,
- GSM8113120 15454, glucose, Replicate 2 15454, glucose,
- GSM8113121 15454, glucose, Replicate 3 15454, glucose,
- GSM8113122 15454, metformin, Replicate 1 15454, metformin,
- GSM8113123 15454, metformin, Replicate 2 15454, metformin,
- GSM8113124 15454, metformin, Replicate 3 15454, metformin,
- GSM8113125 hFOB, galactose, Replicate 1 hFOB, galactose,
- GSM8113126 hFOB, galactose, Replicate 2 hFOB, galactose,
- GSM8113127 hFOB, galactose, Replicate 3 hFOB, galactose,
- GSM8113128 hFOB, glucose, Replicate 1 hFOB, glucose,
+ 58 more — browse all 98 samples with per-sample file links →
- GSE268670 SPRR1A is a potential therapeutic target for osteosarcoma: in vitro and in vivo evaluations using novel generated artificial osteosarcoma cancer stem cell-like cells 3 samples same disease briefing · shares osteosarcoma, vitro
- GSE253548 The whole transcriptome analysis using FFPE and fresh tissue samples identifies the molecular fingerprint of osteosarcoma 90 samples same disease briefing · shares osteosarcoma
- GSE152048 Single cell analysis of osteosarcoma tissues 76 samples same disease briefing · shares osteosarcoma
- GSE317512 Age-stratified transcriptomic profiling reveals biologically distinct molecular phenotypes across pediatric, adolescent, and adult osteosarcoma 70 samples same disease briefing · shares osteosarcoma
- GSE218035 Long-read transcriptome sequencing reveals expression characteristics of osteosarcoma 36 samples same disease briefing · shares osteosarcoma
- GSE293856 Alphavirus M1 disrupts super-enhancer-driven oncogenic transcription in osteosarcoma [RNA-seq] 32 samples same disease briefing · shares osteosarcoma
- GSE126209 Gene expression profiling in Osteosarcomas 23 samples same disease briefing · shares osteosarcoma
- GSE312026 Epigenetic remodeling in sarcoma promotes T cell infiltration via modulation of the Hippo pathway (human) 21 samples same disease briefing · shares modeling
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.