← BioTransfer GEO Dataset Finder
GEO series

Aberrant neurodevelopment in human iPS cell-derived astrocyte-neuron co-culture model of Alexander disease

GSE261110 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2024/04/07 Platform GPL24676
Summary
Co-cultures of neurons and astrocytes derived from human iPS cells carrying a GFAP (R239C) mutation - an identified cause of Alexander disease (AxD) - were analyzed with single-cell RNA sequencing to assess cell population composition, differential gene expression, and cell-cell interaction differences in AxD co-cultures compared to controls. Oxygen-glucose deprivation (OGD) challenge was applied to identify differences in stress response. Our results suggested differentiation impairment especially in AxD astrocytes, represented by an enriched population of less differentiated cells and downregulation of mature astrocyte genes in AxD astrocytes-containing co-cultures. Furthermore, AxD co-cultures showed increased stress response represented by upregulation of metallothioneins and increased susceptibility to the OGD challenge.
Published in
Aberrant neurodevelopment in human iPS cell-derived models of Alexander disease
Matusova Z, Dykstra W, de Pablo Y et al. · Glia 2025 · PMID 39308436 · doi:10.1002/glia.24618
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE261110_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1085307 and SRA study SRP494009. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 6 more — browse all 6 samples with per-sample file links →

Similar datasets

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.