← BioTransfer GEO Dataset Finder
GEO series

Aberrant neurodevelopment in human iPS cell-derived neural organoid model of Alexander disease

GSE261157 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2024/04/07 Platform GPL24676
Summary
3D-cultured unguided neural and cortical organoids derived from human iPS cells carrying a GFAP (R239C) mutation - an identified cause of Alexander disease (AxD) - and their isogenic controls were analyzed with scRNA-seq to investigate the effect of the GFAP mutation on brain development, cell type composition, and gene expression. Results of this analysis showed impaired astro- and neurogenesis in both types of organoids (unguided and cortical), including a lack of cells acquiring the astrocyte fate, and an increased abundance of cells differentiating into lineages other than neuroectodermal. The results also suggested dysregulation of extracellular matrix, membrane, and cytoskeleton components, which might have also affected their differentiation trajectories.
Published in
Aberrant neurodevelopment in human iPS cell-derived models of Alexander disease
Matusova Z, Dykstra W, de Pablo Y et al. · Glia 2025 · PMID 39308436 · doi:10.1002/glia.24618
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE261157_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1085644 and SRA study SRP494112. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 4 more — browse all 4 samples with per-sample file links →

Similar datasets

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.