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Identification and characterization of a human MORC2 DNA binding region that is required for gene silencing

GSE262311 Homo sapiens Expression profiling by high throughput sequencing 9 samples Submitted 2024/12/13 Platform GPL15433
Summary
The eukaryotic microrchidia (MORC) protein family are DNA gyrase, Hsp90, histidine kinase, MutL (GHKL)-type ATPases involved in gene expression regulation and chromatin compaction. The molecular mechanisms underlying these activities are incompletely understood. Here, we studied the full-length human MORC2 protein biochemically. We identified a DNA binding site in the C-terminus of the protein, and we observe that this region can be phosphorylated in cells. DNA binding by MORC2 reduces its ATPase activity and MORC2 can entrap multiple DNA substrates between its N-terminal GHKL and C-terminal coiled coil 3 dimerization domains. Finally, we observe that the MORC2 C-terminal DNA binding region is required for gene silencing in cells. Together, our data provide a model to understand how MORC2 engages with DNA substrates to mediate gene silencing.
Published in
Identification and characterization of a human MORC2 DNA binding region that is required for gene silencing
Fendler NL, Ly J, Welp L et al. · Nucleic acids research 2025 · PMID 39739841 · doi:10.1093/nar/gkae1273
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Also filed as BioProject PRJNA1091320 and SRA study SRP497451. Searching any of these in the dataset finder brings you back here.

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