GEO series

Human mast cell interferon responses and survival in the context of Coronavirus and Respiratory Syncytial Virus are enhanced by IL-5

GSE262880 Homo sapiens (human) RNA-seq (gene expression) 16 samples Submitted 2025/03/17 Platform GPL21697

At a glance

RNA-seq (gene expression) · human · 16 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Homo sapiens (human)
Samples: 16 samples: 8 × CBMCs, IL-5 biol vs 8 × CBMCs, Mock biolGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: IL-5 enhances human mast cell survival and interferon responses to viral infectionThe Journal of allergy and clinical immunology · 2025

Summary (written by the submitters)

Mast cells have been shown to be important sentinel cells in multiple models of viral and bacterial infection. Exacerbations of allergic asthma and asthma deaths are often associated with respiratory viral infections. Elevated levels of type 2 cytokines, especially interleukin 5 (IL-5), are also associated with allergic disease and have been targeted therapeutically.

Read the full summary ↓

Here, we demonstrate that IL-5 signaling enhances the ability of human mast cells to mount a potent anti-viral response against respiratory viruses. Mast cells derived from umbilical cord-blood, treated with IL-5 and infected with respiratory viruses such as the human Coronavirus OC43 or respiratory syncytial virus (RSV) produced significantly more type I and type III interferons (IFNs) than non-IL-5 treated controls. IL-5 treatment also led to greater expression of IFN stimulated genes and upregulated expression of the pro-survival factor B-cell lymphoma 2 (BCL2) and protected mast cells from apoptosis-induced stress. IL-5 also upregulated expression of Endothelial PAS Domain Protein 1 (EPAS1), demonstrating an important IL-5 driven pro-survival network in mast cells. These resultssuggest an unexpected role for IL-5 signaling in regulating mast cell interferon responses and maintaining mast cell populations during mucosal viral infection.

Published in

IL-5 enhances human mast cell survival and interferon responses to viral infection

Barra J, Liwski CR, Phonchareon P et al. · The Journal of allergy and clinical immunology 2025 · PMID 40024310 · doi:10.1016/j.jaci.2025.02.025

This dataset

Open GSE262880 in finder → Find more human RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE262880_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1094574 and SRA study SRP498885. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40024310) ↗

Study design

8 × CBMCs, IL-5 biol vs 8 × CBMCs, Mock biol

Supports a between-group comparison across 16 samples.

How we read this: 2 replicated groups read from 16 sample titles; they account for 16 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8180974 CBMCs, IL-5 biol rep1 CBMCs, IL-5 biol
GSM8180975 CBMCs, Mock biol rep1 CBMCs, Mock biol
GSM8180976 CBMCs, IL-5 biol rep2 CBMCs, IL-5 biol
GSM8180977 CBMCs, Mock biol rep2 CBMCs, Mock biol
GSM8180978 CBMCs, IL-5 biol rep3 CBMCs, IL-5 biol
GSM8180979 CBMCs, Mock biol rep3 CBMCs, Mock biol
GSM8180980 CBMCs, IL-5 biol rep4 CBMCs, IL-5 biol
GSM8180981 CBMCs, Mock biol rep4 CBMCs, Mock biol
GSM8180982 CBMCs, IL-5 biol rep5 CBMCs, IL-5 biol
GSM8180983 CBMCs, Mock biol rep5 CBMCs, Mock biol
GSM8180984 CBMCs, IL-5 biol rep6 CBMCs, IL-5 biol
GSM8180985 CBMCs, Mock biol rep6 CBMCs, Mock biol
GSM8180986 CBMCs, IL-5 biol rep7 CBMCs, IL-5 biol
GSM8180987 CBMCs, Mock biol rep7 CBMCs, Mock biol
GSM8180988 CBMCs, IL-5 biol rep8 CBMCs, IL-5 biol
GSM8180989 CBMCs, Mock biol rep8 CBMCs, Mock biol

Datasets on a similar topic

GSE309353 Respiratory syncytial virus nonstructural protein 1 inhibits secretion of cytokines and chemokines by differentiated primary nasal epithelial cells cultured at air-liquid interface 24 samples shares respiratory, syncytial
GSE268742 Respiratory syncytial virus (RSV) enhances translation of virus-resembling AU-rich host transcripts 12 samples shares respiratory, syncytial
GSE232687 Respiratory Syncytial virus (RSV) 44 samples shares respiratory, syncytial
GSE309140 Enhanced interferon-stimulated gene signatures in B cells following influenza B virus infection compared to influenza vaccination 16 samples shares interferon, enhanced
GSE298740 Transcriptomics in T2-high severe asthmatics treated with biologics: Comparison between anti-IgE, anti-IL-5 and anti-IL-5R-targeted treatments. 70 samples shares il-5
GSE285468 IL-5 increases pro-fibrotic and pro-inflammatory signalling in severe asthmatic fibroblasts 12 samples shares il-5
GSE282464 Molecular landscape of respiratory infection: A large-scale, multi-centre blood transcriptome dataset 377 samples shares respiratory
GSE317890 Transcriptomic profiling of interferon responses induced by IVT sgRNAs in CRISPRi HEK293T cells 360 samples shares interferon

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.