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Comparative framework and adaptation of ACME HS approach to single cell isolation from fresh-frozen endocrine tissue [dataset 1]

GSE263061 Homo sapiens Expression profiling by high throughput sequencing 9 samples Submitted 2025/01/08 Platform GPL24676
Summary
Current dissociation methods for scRNA-seq studies of solid tissues do not guarantee intact single-cell isolation from fresh frozen samples, especially for sensitive and heterogeneous endocrine tissues. Here, we adapted the acetic-methanol dissociation method – ACME High Salt (ACME HS) to isolate intact single cells from fresh-frozen endocrine tumor samples. We compared enzymatic, ACME HS, and nuclear isolation methods ability to preserve major cell types and gene expression. We demonstrated that ACME HS dissociates and fixes cells, preserving cell morphology and high RNA integrity. This renders ACME HS a valuable alternative in the scRNA-seq protocols for challenging tissues.
Published in
Comparative evaluation of ACetic - MEthanol high salt dissociation approach for single-cell transcriptomics of frozen human tissues
Utkina M, Shcherbakova A, Deviatiiarov R et al. · Frontiers in cell and developmental biology 2024 · PMID 39839668 · doi:10.3389/fcell.2024.1469955
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Also filed as BioProject PRJNA1095526 and SRA study SRP499361. Searching any of these in the dataset finder brings you back here.

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