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Next Generation Sequencing Facilitates Quantitative Analysis of wild type and KAT8-deficient Bone Marrow Derived macrophage Transcriptomes

GSE263149 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2026/02/11 Platform GPL24247
Summary
Purpose: The purpose of this study is to detect activated or silenced genes during wild type (WT) and KAT8-deficient bone marrow derived macrophages (BMDMs). Gene expression differences between two samples could be found using transcriptome profiling (RNA-seq) analysis. Methods: Mouse BMDMs were generated from bone marrow cells in RPMI-1640 medium with recombinant mouse M-CSF (20ng/ml). BMDMs were stained to confirm the surface expression of CD11b and F4/80. Cells with purity >97.5% were used for subsequent experiments. WT and KAT8-deficient BMDMs were stimulated with R848 (100ng/ml) for 0 and 2 hours, of which RNA profiles were generated by deep sequencing, using Illumina. Results: We mapped about 10 million sequence reads per sample to the mouse genome, identified hundreds of genes with significant mRNA between WT and KAT8-deficient BMDMs.
Published in
Targeting KAT8 alleviates self-RNA-driven skin inflammation by modulating histone H4 lysine 16 acetylation in psoriasis
Xiang Y, Jiang Y, Wang Z et al. · Cell death and differentiation 2026 · PMID 40691280 · doi:10.1038/s41418-025-01547-y
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Also filed as BioProject PRJNA1095981 and SRA study SRP499646. Searching any of these in the dataset finder brings you back here.

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