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Single cell RNA sequencing of mutant bone marrow stromal cells lacking HIF2 and controls.

GSE263228 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2024/11/12 Platform GPL24247
Summary
Osteoblast lineage cells within adult bone marrow are exposed to varying oxygen levels. Hypoxia-Inducible Factor-1α (HIF1α) and HIF2α are pivotal in the cellular response to hypoxia. Our study explores the effects of targeted HIF2α deletion in mesenchymal progenitors and their descendants. We demonstrate that HIF2α acts as a negative regulator of osteoblastogenesis and bone mass accrual. Therapeutically targeting HIF2α could be beneficial in treating low bone mass conditions, such as those observed in chronic diseases, osteoporosis, or during aging. To elucidate the mechanisms underlying the increased bone mass resulting from HIF2α loss, we performed single-cell RNA sequencing (scRNA-seq) on bone marrow stromal cells from both mutant mice lacking HIF2α in PRX1 lineage cells and control mice.
Published in
Pharmacological inhibition of HIF2 protects against bone loss in an experimental model of estrogen deficiency
Lanzolla G, Sabini E, Beigel K et al. · Proceedings of the National Academy of Sciences of the United States of America 2024 · PMID 39602268 · doi:10.1073/pnas.2416004121
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Also filed as BioProject PRJNA1095970 and SRA study SRP499558. Searching any of these in the dataset finder brings you back here.

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