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Rational cell fate engineering through chromatin dynamics [CUT&TAG II]

GSE263597 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 8 samples Submitted 2024/06/24 Platform GPL24247
Summary
Mechanism controlling cell fate remains elusive. Chromatin remodeling complex interacts with transcription factors to colocalize across the genome and regulate region-specific epigenetic environment. Here, we propose an engineering approach for controlling cell fate through chromatin closing and opening. We utilize chromatin remodeling complex, BAF, known to activate gene expression by opening chromatin loci. By grafting BAF interacting motifs onto Nanog, we show that engineering factors could promote somatic cell reprogramming with Oct4. Furthermore, mutation on the interacting motifs render iPSC generation. The syntactic factors facilitate cell fate transition by recruiting BAF complex to modulate chromatin accessibility and reorganize cell state specific enhancers. Our findings reveal alternative methods to control cell fate by manipulating chromatin accessibility.
Published in
Engineering mouse cell fate controller by rational design
Huang T, Liu D, Wang X et al. · Nature communications 2024 · PMID 39043686 · doi:10.1038/s41467-024-50551-2
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Also filed as BioProject PRJNA1098231 and SRA study SRP500596. Searching any of these in the dataset finder brings you back here.

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