Synergistic effect of IL-33 priming and ATP stimulation in bone marrow derived mast cells

Synergy of signals often causes cells to exhibit stronger physiological responses, especially immune cells. Here we reported that priming of interleukin-33 (IL-33) played a crucial role in the subsequent response of mast cells to ATP. Judging from the levels of cytokines and chemokines, the synergy of IL-33 priming and ATP stimulation was as high as 64%. At the entire transcriptional level, high synergy was also observed. This provided evidence supporting the sequential release of IL-33 and ATP derived from stromal cells on mast cells in vivo. Likewise, the upregulation of numerous cytokines and chemokines provided enormous support for mast cells to regulate antiviral immunity.