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The interactive effect of sustained sleep restriction and resistance exercise on skeletal muscle transcriptomics in young females

GSE263838 Homo sapiens (human) RNA-seq (gene expression) 38 samples Submitted 2025/04/12 Platform GPL24676
At a glance

RNA-seq (gene expression) · human · 38 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
38 samples: 10 conditions, mostly with about 4 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
The interactive effect of sustained sleep restriction and resistance exercise on skeletal muscle transcriptomics in young femalesPhysiological genomics · 2024
Summary (written by the submitters)
Both sleep loss and exercise regulate gene expression in skeletal muscle, yet little is known about how the interaction of these stressors affects the transcriptome. The aim of this study was to investigate the effect of nine nights of sleep restriction, with repeated resistance exercise (REx), on the skeletal muscle transcriptome of young, trained females.
Published in
The interactive effect of sustained sleep restriction and resistance exercise on skeletal muscle transcriptomics in young females
Knowles OE, Soria M, Saner NJ et al. · Physiological genomics 2024 · PMID 38766755 · doi:10.1152/physiolgenomics.00010.2024
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE263838_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1099582 and SRA study SRP501408. Searching any of these in the dataset finder brings you back here.

Study design
10 conditions, mostly with about 4 replicates each
ANLI ×4 ELLI ×4 GEDA ×4 GEKN ×4 HABA ×4 JEHA ×4 KISZ ×4 NAC ×4 +2 more

Supports a between-group comparison across 38 samples.

How we read this: 10 replicated groups read from 38 sample titles; they account for 38 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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