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RNA binding protein PCBP2 regulates pancreatic β cell function and adaptation to glucose

GSE263908 Mus musculus (mouse) RNA-seq (gene expression) 17 samples Submitted 2024/06/17 Platform GPL17021
At a glance

RNA-seq (gene expression) · mouse · 17 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
17 samples: 5 × PCBP2KO β cells, 2.8mM G, vs 5 × PCBP2KO β cells, 16.7mM G, vs 4 × WT β cells, 16.7 mM G, vs 3 × WT β cells, 2.8mM G,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Glucose plays a key role in shaping pancreatic β cell function. Thus, deciphering the mechanisms by which this nutrient stimulates β cells holds therapeutic promise for combating β cell failure in type 2 diabetes (T2D). β cells respond to hyperglycemia in part by rewiring their mRNA metabolism, yet the factors governing these changes remain poorly understood. Here, we interrogate the transcriptomes of control β cells and mutant β cells lacking the RNA binding protein PCBP incubated with low or high glucose for 72 hours.
This dataset
Open GSE263908 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE263908_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1099898 and SRA study SRP501605. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
5 × PCBP2KO β cells, 2.8mM G, vs 5 × PCBP2KO β cells, 16.7mM G, vs 4 × WT β cells, 16.7 mM G, vs 3 × WT β cells, 2.8mM G,

Supports a between-group comparison across 17 samples.

How we read this: 4 replicated groups read from 17 sample titles; they account for 17 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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