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IRF7 promotes systemic autoimmunity by transcriptionally regulating B cell differentiation through germinal center and plasma cell pathways [scRNA-seq]

GSE264033 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2025/03/01 Platform GPL24247
Summary
Little is known about how interferon regulatory factor 7 (IRF7) promotes systemic autoimmunity. Here performed scRNAseq on B cells purified from the spleens of 3 month old FcgRIIB-/-IRF7-/- and FcgRIIB-/- controls. We identified a role for IRF7 in promoting the differentiation of B cells through the GC and PC pathways during an autoimmune response. To determine which genes IRF7 specifically controlled for this response, we purified class-switched activated B cells from FcgRIIB-/- mice and performed IRF7 ChIPseq. We identified that IRF7 transcirptionally regulated genes assocaited with B cell protein translation and metabolism. In summary, we have identified a B cell intrinsic role for IRF7 in driving the transcriptoinal profile required for GC and PC differentiation in SLE-like autoimmunity.
Published in
IRF7 controls spontaneous autoimmune germinal center and plasma cell checkpoints
Fike AJ, Bricker KN, Gonzalez MV et al. · bioRxiv : the preprint server for biology 2025 · PMID 39974943 · doi:10.1101/2025.02.04.636277
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Also filed as BioProject PRJNA1100580 and SRA study SRP501942. Searching any of these in the dataset finder brings you back here.

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