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Pcbp1 is crucial for Naïve-to-Primed pluripotency transition due to regulation of amino acid metabolism [ChIP-seq]

GSE264036 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2026/06/04 Platform GPL21103Platform GPL24247
Summary
Embryo implantation coincides with the Naïve-to-Primed pluripotency transition. From fertilization to implantation only a number of cleavages is observed without significant embryo growth. Embryo-uterine connection provides necessary nutrients for further development while metabolism intensification is occurred. We have found that in vitro Naïve-to-Primed pluripotency transition of ESCs with Pcbp1 knockout is worsened – reduction of proliferation and cell death is observed. By using ChIP-seq, RNA-seq and Mass-spectrometry we show that Pcbp1 is detrimental for pluripotent properties, but is crucial for amino acid metabolism intensification. According to this, functional approaches showed protein synthesis decline, while embryos deficient for Pcbp1 demonstrated growth absence after implantation.
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Direct links to NCBI, no account and no request form: the whole study as GSE264036_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1100583 and SRA study SRP501909. Searching any of these in the dataset finder brings you back here.

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