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Identifying differences in differentiation between adherent and suspension culture for iPSC derived beta cells

GSE264176 Homo sapiens Expression profiling by high throughput sequencing 9 samples Submitted 2024/04/26 Platform GPL15520
Summary
Stage 3 cells from a suspension environment and adherent culture were compared and evaluated through RNA sequencing. RNA sequencing data in heatmap and volcano plot evaluation of core endocrine genes showed that expression levels in adherent cells were lower than those expressed in 3D environment. Several genes that are essential for insulin transcription such as NEUROD1, MAFA, PPARGC1A and NKX2.2 along with INS were expressed with higher transcript levels in bioreactor samples. It was observed that genes usually expressed in earlier stages such as FOXA2 and HNF1b had higher expression levels in adherent culture indicating that 2D environment differentiation might be lagging.
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Direct links to NCBI, no account and no request form: the whole study as GSE264176_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 9 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1101164 and SRA study SRP502168. Searching any of these in the dataset finder brings you back here.

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