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Single nucleus RNA sequencing of hippocampal tissue from wildtype and SHIP1 conditional knockout mice at postnatal day 15.

GSE264452 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2025/04/28 Platform GPL30172
Summary
This study corresponds to the single nucleus RNA sequencing (snRNAseq) analysis done as part of the manuscript "Microglial SHIP1 controls synaptic pruning in the developing hippocampus via the complement system" by Matera et al. INPP5D, which encodes the lipid phosphatase SHIP1, is one of the most common genes associated with the risk of Alzheimer’s disease and is enriched in microglia in the central nervous system. SHIP1 has been found to be highly expressed in plaque-associated microglia. However, how it regulates microglial function and influences brain physiology has been poorly investigated. In this study, we generated a snRNAseq dataset from the hippocampi of wildtype (WT) and conditional SHIP1 knockout (KO) mice at postnatal day 15. By predicting cell-cell communication, we found decreased neuronal interactions in KO mice, in line with results of synaptic loss in the rest of the manuscript.
Published in
Microglial lipid phosphatase SHIP1 limits complement-mediated synaptic pruning in the healthy developing hippocampus
Matera A, Compagnion AC, Pedicone C et al. · Immunity 2025 · PMID 39657671 · doi:10.1016/j.immuni.2024.11.003
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Direct links to NCBI, no account and no request form: the whole study as GSE264452_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1102428 and SRA study SRP502938. Searching any of these in the dataset finder brings you back here.

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