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Transcriptional effects of co-culture on the spatiotemporal dynamics of T cell motility and cancer-T cell interactions [scRNA-seq]

GSE264478 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2024/04/26 Platform GPL24247
Summary
Insufficient infiltration of cytotoxic T cells into solid tumors poses a major challenge in cancer immunotherapy, largely due to the intricate tumor microenvironment. To address this, we co-cultured mouse cancer cell lines (B16-WT or B16-OVA) with cancer-specific cytotoxic T cells (activated OT-1) in vitro to uncover the impact of cancer-T cell interactions on T cell motility, pivotal for effective tumor infiltration. To investigate the potential molecular mechanisms underlying T cell motility patterns in the two coculture contexts, we performed both bulk and single-cell RNA sequencing of cancer and T cells sorted from the co-culture systems at specific time points.
Published in
Deciphering antigen-specific T cell navigation tactics and cancer immune evasion in co-cultures
Li X, Jin T, Wang L et al. · Communications biology 2025 · PMID 40745217 · doi:10.1038/s42003-025-08568-w
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Also filed as BioProject PRJNA1101662 and SRA study SRP502802. Searching any of these in the dataset finder brings you back here.

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