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Influence of Staphylococcus aureus biofilm infection phase on functional composition of host neutrophils

GSE264548 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2024/07/03 Platform GPL21626
Summary
Nosocomial infections resulting from growing biofilms on the surface of indwelling medical devices represent a major therapeutic challenge in an aging population. In this context, current models have so far not addressed the synergy between a developing biofilm and the host’s immune response. Here we employed a mouse model for implant-associated infection from Staphylococcus aureus biofilms and, through functional assays, next generation single cell sequencing and spectral flow cytometry, observed a direct influence of the developing biofilms in the phenotype of tissue infiltrating neutrophils over the course of infection. Our results allowed us to differentiate neutrophil subpopulations, identifying some which may be protective of the biofilm and which could be the target for future therapies.
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Direct links to NCBI, no account and no request form: the whole study as GSE264548_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1103133 and SRA study SRP503335. Searching any of these in the dataset finder brings you back here.

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