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RNA-seq-based transcriptome profiling of fruit extract of Brucea javanica (FEBJ) and cynaroside

GSE266353 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2025/03/12 Platform GPL24676
Summary
Liver fibrosis is a wound-healing process that occurs in response to chronic liver injury. Traditional Chinese medicine (TCM) has been shown to have a significant effect on treating liver fibrosis. We found that the fruit extract of Brucea javanica (FEBJ) suppressed hepatic stellate cell (HSC) activation and extracellular matrix (ECM) deposition in vitro, and attenuated thioacetamide (TAA)- induced liver fibrosis in zebrafish. Moreover, we identified cynaroside as one of the active compounds of FEBJ that could reproduce its antifibrotic effects in vitro and in vivo. To further investigate the global transcriptome changes of FEBJ and cynaroside, RNA-seq was performed. The results indicated that FEBJ-regulated genes were involved mainly in ECM-remodeling related biological functions, and cynaroside-regulated genes were involved in various biological functions besides ECM-remodeling.
Published in
Identification of the fruit of Brucea javanica as an anti-liver fibrosis agent working via SMAD2/SMAD3 and JAK1/STAT3 signaling pathways
Yan D, Qiao L, Huang W et al. · Journal of pharmaceutical analysis 2025 · PMID 40034864 · doi:10.1016/j.jpha.2024.101047
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Also filed as BioProject PRJNA1106812 and SRA study SRP505166. Searching any of these in the dataset finder brings you back here.

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