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KAP1 negatively regulates elongation kinetics to activate signal-induced transcription [TT-Seq]

GSE266694 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2024/05/07 Platform GPL30173
Summary
Signal-induced transcriptional programs regulate critical biological processes through the precise spatiotemporal activation of Immediate Early Genes (IEGs); however, the mechanisms of transcription induction are still unfolding. By combining an acute depletion system with high resolution genomics approaches to interrogate synchronized, temporal transcription, we reveal that KAP1/TRIM28 is a first responder that fulfills the temporal and heightened transcriptional demand of IEGs. Unexpectedly, acute KAP1 loss triggers an increase in RNA Polymerase II elongation kinetics during early stimulation time points. This elongation defect derails the normal progression through the transcriptional cycle during late stimulation time points, ultimately leading to decreased recruitment of the transcription apparatus for re-initiation and dampening IEG transcription. Collectively, KAP1 plays a counterintuitive role by negatively regulating transcription elongation to support full activation across multiple transcription cycles of genes critical for cell physiology and organismal functions.
Published in
KAP1 negatively regulates RNA polymerase II elongation kinetics to activate signal-induced transcription
Hyder U, Challa A, Thornton M et al. · bioRxiv : the preprint server for biology 2024 · PMID 38746145 · doi:10.1101/2024.05.05.592422
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Also filed as BioProject PRJNA1108274 and SRA study SRP506478. Searching any of these in the dataset finder brings you back here.

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