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Blockade of Endothelin Receptors Mitigates SARS-CoV-2-induced Osteochondral Damage

GSE267009 Homo sapiens Expression profiling by high throughput sequencing 9 samples Submitted 2024/05/08 Platform GPL24676
Summary
Previous studies have detected the presence of SARS-CoV-2 viral proteins in bronchial cartilage chondrocytes. We hypothesised that the leakage of viral proteins to local joint tissue was due to virus-induced endothelial dysfunction. Studies have also shown upregulation of endothelin-1 (ET-1), the most potent vasoconstrictor, in COVID patients. We are investigating the direct effect of SARS-CoV-2 spike protein (SP) and the host response to chondrocytes. Human mesenchymal stem cells (hMSCs)-differentiated chondrocytes were treated with either full-length SARS-CoV-2 spike protein (SP) only or a combination of spike protein, neutralising antibody to S1 and endothelin-1 (SAE) to mimic viral insult and host response respectively. RNA sequencing was performed to compared the change in transcriptome in control, SP and SAE. All samples were processed in the same batch. Default quality control parameters were used.
Published in
Blockade of endothelin receptors mitigates SARS-CoV-2-induced osteoarthritis
Au MT, Ni J, Tang K et al. · Nature microbiology 2024 · PMID 39261580 · doi:10.1038/s41564-024-01802-x
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Also filed as BioProject PRJNA1104007 and SRA study SRP503723. Searching any of these in the dataset finder brings you back here.

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