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Gene expression profile at single cell level of cells from whole lung to assess MAGE-A4 expressing tumor alterations in tumor microenvironment

GSE267420 Mus musculus Expression profiling by high throughput sequencing 5 samples Submitted 2025/02/19 Platform GPL24247
Summary
Melanoma Antigen-A4 (MAGE-A4) is a common cancer testis antigen expressed in human non-small cell lung cancer; however, whether and how it contributes to cancer initiation and progression has remained less clear. We engineered mice to express human MAGE-A4 specifically in Club cells of the bronchial epithelium, with or without loss of Pten in the same cells, to investigate the role of MAGE-A4 in tumor initiation and development. We used single cell transcriptomics and flow cytometry to evaluate the immune and other cells in the lung/lung tumor microenvironment that were altered in response to this cancer testis antigen. We found that endothelial cells in MAGE-A4 expression models upregulate CXCL12, which likely contributes to the increased plasma cell recruitment to the lungs infiltrating MAGE-A4-expressing lung tumors in both mouse and human. Single cells were isolated from whole lung of mice at 5 months of age when tumor had developed in MAG4/Pt lungs and were analyzed using scRNA-seq.
Published in
MAGE-A4 induces non-small cell lung cancer and tumor-promoting plasma cell accumulation
Armstrong D, Chang CY, Hong MJ et al. · Science advances 2025 · PMID 39937892 · doi:10.1126/sciadv.ads4227
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Also filed as BioProject PRJNA1111486 and SRA study SRP507590. Searching any of these in the dataset finder brings you back here.

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