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Exploring the utility of snRNA-seq in profiling human bladder tissue: A comprehensive comparison with scRNA-seq

GSE267964 Homo sapiens Expression profiling by high throughput sequencing 8 samples Submitted 2024/11/18 Platform GPL24676
Summary
Single cell sequencing technologies have revolutionized our understanding of biology by mapping cell diversity and gene expression in healthy and diseased tissues. While single-cell RNA sequencing (scRNA-seq) has been widely used, interest in single-nucleus RNA sequencing (snRNA-seq) is growing due to its benefits, including the ability to analyze archival tissues and capture rare cell types that are challenging to dissociate. However, comparative studies across tissues have yielded mixed results, with some reporting enhanced cell type retention using snRNA-seq while others finding cell type identification to be challenging in snRNA-seq data. The GUDMAP consortium aims to construct a molecular atlas of the lower urinary tract (LUT); thus, we set out to determine the strengths and limitations of each approach in characterizing LUT cell types. Using the human bladder, we determined that scRNA-seq offered more discriminative gene sets for identification while snRNA-seq could facilitate capture of previously underrepresented cell types.
Published in
Exploring the utility of snRNA-seq in profiling human bladder tissue: A comprehensive comparison with scRNA-seq
Santo B, Fink EE, Krylova AE et al. · iScience 2025 · PMID 39850354 · doi:10.1016/j.isci.2024.111628
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Also filed as BioProject PRJNA1111560 and SRA study SRP507629. Searching any of these in the dataset finder brings you back here.

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