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Single-cell Transcriptome Analysis Reveals Mechanical Properties of Leukemic Stem Cells in AML.

GSE267998 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2025/10/10 Platform GPL24676
Summary
Leukemic stem cells (LSCs) are crucial drivers of leukemia, yet the mechanisms underlying their function remain poorly understood. Here, we investigated the physical properties of LSCs and their potential role in LSCs function. single-cell RNA-sequencing analysis has revealed that small-sized LSCs aberrantly overexpress ALDH1A1, a crucial regulator of stem cell function. Lastly, ALDH1A1 inhibition increases cell stiffness and suppresses leukaemogenesis in patient-derived xenograft models. Our findings establish a novel link between the biomechanics of LSCs and their function in leukemia, providing a compelling rationale for targeting therapy-resistant LSCs by inhibiting G3BP. Overall, our study provides valuable insights into the mechanisms of LSCs function and has significant implications for the development of more effective therapies for this devastating disease.
Published in
Targeting leukemic stem cell biomechanics suppresses stemness and enhances NK cell-mediated immunotherapy
Zhu M, Yang H, Qiu K et al. · Nature communications 2025 · PMID 41326331 · doi:10.1038/s41467-025-65842-5
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Also filed as BioProject PRJNA1114012 and SRA study SRP508959. Searching any of these in the dataset finder brings you back here.

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