A NAD + Metabolic Checkpoint Regulates Hematopoietic Stem Cell Activation and Aging
RNA-seq (gene expression) · mouse · 12 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Mus musculus (mouse)
- Samples
- 12 samples: 4 × HSC, young, homeostasis, vs 4 × HSC, young, ex vivo, vs 4 × HSC, old, homeostasis,Groups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- An NAD(+)-dependent metabolic checkpoint regulates hematopoietic stem cell activation and agingNature aging · 2024
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE268310_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1116149 and SRA study SRP509796. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 12 samples.
How we read this: 3 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8289512 HSC, young, homeostasis, 1 HSC, young, homeostasis,
- GSM8289513 HSC, young, homeostasis, 2 HSC, young, homeostasis,
- GSM8289514 HSC, young, homeostasis, 3 HSC, young, homeostasis,
- GSM8289515 HSC, young, homeostasis, 4 HSC, young, homeostasis,
- GSM8289516 HSC, young, ex vivo, 1 HSC, young, ex vivo,
- GSM8289517 HSC, young, ex vivo, 2 HSC, young, ex vivo,
- GSM8289518 HSC, young, ex vivo, 3 HSC, young, ex vivo,
- GSM8289519 HSC, young, ex vivo, 4 HSC, young, ex vivo,
- GSM8289520 HSC, old, homeostasis, 1 HSC, old, homeostasis,
- GSM8289521 HSC, old, homeostasis, 2 HSC, old, homeostasis,
- GSM8289522 HSC, old, homeostasis, 3 HSC, old, homeostasis,
- GSM8289523 HSC, old, homeostasis, 4 HSC, old, homeostasis,
- GSE297515 Piezo1-dependent activation of stromal cells ignites muscle inflammation in exercise and injury and is associated with inflammaging 90 samples shares activation, aging
- GSE281467 Coactivation of innate immune suppressive cells induces acquired resistance against cancer immunotherapy targeting the innate immune system [TIICs RNA-seq] 30 samples shares activation, stem
- GSE306935 The Integrated Stress Response Pathway Improves Aged Murine Muscle Stem Cell Activation and in vivo Regeneration 12 samples shares activation, stem
- GSE222941 Endomucin marks quiescent long-term multi-lineage repopulating hematopoietic stem cells and is essential for their transendothelial migration [RNA-seq: wildtype_EMCN] 11 samples shares hematopoietic, stem
- GSE247504 Sustained NF-kB activation allows mutant alveolar stem cells to co-opt a regeneration program for tumor initiation [Expt2] 10 samples shares activation, stem
- GSE277443 EMP1 preserves hematopoietic stem cell potential under homeostasis and radiation-induced injury by alleviating ER stress [ER-stress RNA-seq] 10 samples shares hematopoietic, stem
- GSE342096 Distinct activation programs in naïve and memory CD8 T cells govern progeny stemness and effector persistence 37 samples shares activation, stem
- GSE277681 Premature senescence impairs hematopoietic stem cell function during sickle cell disease in mice and humans (Mouse CITE-Seq) 32 samples shares hematopoietic, stem
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.