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Effects of envelope- or membrane-protein segments of SARS-CoV-2 on gene expression of HUVEC cells

GSE268369 Homo sapiens Expression profiling by high throughput sequencing 9 samples Submitted 2024/07/01 Platform GPL28038
Summary
Exterior segments of E-proteins bound onto and modulated gene expression in human vascular endothelial cells in vitro The finding that EC38 segment laid out of SARS-CoV-2 virus envelope prompted us to wonder if this segment would bind onto and impact the biology of host cells. Bearing the viremia during severe COVID-19 in mind, more attention was paid to the circulation system. Cultured human umbilical vein endothelial cells (HUVECs) were used to test the binding of EC38 and MN19 peptides and the potential consequence in terms of gene expression upon such binding. To check if the binding of interest peptides onto HUVEC concurred functional consequences in the cells, HUVECs were treated with EC38 and MN19 peptides (5μM) for 24 hours, and their transcriptome was measured via RNA sequencing.
Published in
C-terminus-outward orientation of SARS-CoV-2 envelope proteins on viral capsid enables a novel virus-cell interaction pathway
Xu J, Zhao W, Li Y et al. · Frontiers in cellular and infection microbiology 2026 · PMID 41909841 · doi:10.3389/fcimb.2026.1776252
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Also filed as BioProject PRJNA1116728 and SRA study SRP509972. Searching any of these in the dataset finder brings you back here.

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