RNA-seq analysis to examine the effect of Jmjd1a knockdown in 3T3-L1 pre-adipocyte cells

Adipocyte differentiation has been shown to require iron, but the underlying mechanism remains unclear. We have previously reported that iron supply through ferritinophagy is associated with demethylation of repressive histone marks in the differentiation-associated genes, which is mediated by the histone demethylase JMJD1A. To examine how JMJD1A regulates the gene expression program during adipocyte differentiation, in this study we peformed RNA-seq analysis using 3T3-L1 pre-adipocytes with Jmjd1a knockdown.