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ASCT2 is a major contributor to serine uptake in cancer cells

GSE269606 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2024/06/11 Platform GPL24676
Summary
The non-essential amino acid serine is a critical nutrient for cancer cells due to its diverse biosynthetic functions. While some tumors can synthesize serine de novo, others are auxotrophic and therefore reliant on serine uptake. Importantly, despite several transporters being known to be capable of transporting serine, the transporter(s) that mediate serine uptake in cancer cells are not known. Here, we characterize the amino acid transporter ASCT2 (SLC1A5) as a major contributor to serine uptake in cancer cells. ASCT2 is well-known as a glutamine transporter in cancer, and our work demonstrates that serine and glutamine compete for uptake through ASCT2. We further show that ASCT2-mediated serine uptake is essential for purine nucleotide biosynthesis and that ERα promotes serine uptake by directly activating SLC1A5 transcription. Together, our work defines an additional important role for ASCT2 as a serine transporter in cancer and evaluates ASCT2 as a potential therapeutic target.
Published in
ASCT2 is the primary serine transporter in cancer cells
Conger KO, Chidley C, Ozgurses ME et al. · bioRxiv : the preprint server for biology 2023 · PMID 37873453 · doi:10.1101/2023.10.09.561530
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Also filed as BioProject PRJNA1122640 and SRA study SRP513242. Searching any of these in the dataset finder brings you back here.

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