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Neddylation regulate the development of glutamatergic neurons

GSE269898 Mus musculus (mouse) RNA-seq (gene expression) 23 samples Submitted 2025/09/17 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 23 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
23 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Neddylation regulates the development and function of glutamatergic neuronsCommunications biology · 2025
Summary (written by the submitters)
The development and function of synapses is orchestrated by a plethora of regulatory mechanisms hierarchically controlling gene expression to protein function. Expanding the proteome, protein post-translational modification are additional key events in neu-rons.
Read the full summary ↓
In such context, the modification of protein with ubiquitin-like modifier (Ubls) such as the SUMOs, but also other Ubls like NEDD8, have been revealed as major steps in regulating neuronal development and synaptic transmission, either by directly acting lo-cally at synapse or via nuclear-mediated mechanism and the regulation of gene expres-sion. In the present work, we focus on NEDD8 and characterized neuronal morphology, synap-tic transmission and the transcriptional landscape of NEDD8 deficient hippocampal au-taptic neurons. While we observed no major alteration in neuronal morphology, NEDD8-deficient neurons have a decreased pool of ready-releasable synaptic vesicles. This de-fect was likely caused by an imbalance in the expression of vGLUT1/vGLUT2 and de-creased endophilin1 level. Indeed, transcriptome analysis revealed profound defects in the expression of key transcriptional regulators determining glutamatergic neurodevel-opment, indicating that NEDD8 foster the expression of glutamatergic cell fate.
Published in
Neddylation regulates the development and function of glutamatergic neurons
Torres J, Vural Z, Fiosins M et al. · Communications biology 2025 · PMID 40925985 · doi:10.1038/s42003-025-08680-x
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE269898_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1124090 and SRA study SRP514010. Searching any of these in the dataset finder brings you back here.

Samples in this study

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+ 23 more — browse all 23 samples with per-sample file links →

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