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Rapid and efficient generation of human iPSC-derived lymphatic endothelial cells for functional assays

GSE270293 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2025/04/23 Platform GPL34284
Summary
The lymphatic system plays a crucial role in fluid homeostasis, immune responses, and lipid metabolism. Comprising specialized lymphatic endothelial cells (LECs), it enables interstitial fluid uptake and transit to lymph nodes. Investigations of lymphatic vasculature are typically performed in vivo due to the complex nature of this system. However, in vitro models, particularly those using induced pluripotent stem cells (iPSCs), offer a promising alternative by providing a renewable, animal-free, patient-specific, human cell source that can be easily manipulated. Here, we describe a robust and efficient 10-day protocol to differentiate human iPSCs into functional LECs, validated by immunostaining, flow cytometry, and RNA-sequencing. The iPSC-derived LECs (iLECs) exhibit comparable functionality to in vivo lymphatics, including their response to inflammatory and lymphangiogenic stimuli and ability to form 3D lumenized lymphatic networks. The protocol's reproducibility across multiple iPSC lines underscores its reliability and utility for studying lymphatic biology.
Published in
Age-related meningeal extracellular matrix remodeling compromises CNS lymphatic function
Hitpass Romero K, Stevenson TJ, Smyth LCD et al. · Journal of neuroinflammation 2025 · PMID 40247257 · doi:10.1186/s12974-025-03436-0
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Direct links to NCBI, no account and no request form: the whole study as GSE270293_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1126144 and SRA study SRP515567. Searching any of these in the dataset finder brings you back here.

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