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Foxd1 expression identifies a distinct subset of hepatic stellate cells involved in liver fibrosis

GSE270404 Mus musculus (mouse) RNA-seq (gene expression) 12 samples Submitted 2025/06/17 Platform GPL21273
At a glance

RNA-seq (gene expression) · mouse · 12 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
12 samples: 3 × HSC vs 3 × HSC_Tom vs 3 × HSC_CCl4 vs 3 × HSC_CCl4_TomGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
FoxD1 expression identifies a distinct subset of hepatic stellate cells involved in liver fibrosisBiochemical and biophysical research communications · 2024
Summary (written by the submitters)
Hepatic stellate cells (HSCs) are pericytes of the liver and are responsible for liver fibrosis and cirrhosis, which are end stages of chronic liver diseases. TFG-β activates HSCs leading to differentiation of myofibroblasts in the process of liver fibrosis. While heterogeneity of HSCs is appreciated in the fibrotic liver, it remains elusive which HSC subsets mainly contribute to fibrosis.
Read the full summary ↓
We here show that expression of pericyte marker, Foxd1, specifically marked a subset of HSCs in the Foxd1-fate tracer mice. HSCs fate-mapped by Foxd1 were preferentially localized in portal area and peripheral zone of normal liver and fibrotic liver induced by carbon tetrachloride. Furthermore, the deletion of Cbfβ, which is necessary for TGF-β signaling, in Foxd1-expressing cells ameliorated liver fibrosis. Thus, we identified an HSC subset which preferentially responds to liver injuries.
Published in
FoxD1 expression identifies a distinct subset of hepatic stellate cells involved in liver fibrosis
Yamagata K, Takasuga S, Tatematsu M et al. · Biochemical and biophysical research communications 2024 · PMID 39226736 · doi:10.1016/j.bbrc.2024.150632
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE270404_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1126310 and SRA study SRP515116. Searching any of these in the dataset finder brings you back here.

Study design
3 × HSC vs 3 × HSC_Tom vs 3 × HSC_CCl4 vs 3 × HSC_CCl4_Tom

Supports a between-group comparison across 12 samples.

How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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