GEO series

The use of microRNA mimics to increase microRNA levels in the developing organs of newborn mice.

GSE270460 Mus musculus (mouse) RNA-seq (gene expression) 18 samples Submitted 2026/07/13 Platform GPL30172

At a glance

RNA-seq (gene expression) · mouse · 18 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 18 samples
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

Summary (written by the submitters)

MicroRNA are emerging of important regulators of cell and tissue differentiation, organ development, and disease pathogenesis. The use of microRNA antimiRs which deplete microRNA pools is a well-established and successful intervention in both in vivo and in vitro settings, where the goal is to reduce the abundance of selected microRNA species.

Read the full summary ↓

However, whilst efforts to increase the abundance of selected microRNA species in vitro have found widespread success in cell culture, delivering functional microRNA to the organs of living, intact experimental animals remains problematic. The objective of this study was to examine the efficacy of delivery of commercially-available microRNA mimics that are marketed for in vitro and in vivo use to multiple organs of the laboratory mouse, following parenteral administration in various protocols over the first 14 days of post-natal life. The data presented indicate that parenteral administration of a microRNA mimic is largely inefficient, with moderate delivery of a miR-497a-5p mimic noted in the liver, but not the kidneys, lungs, heart, or brain. Changes in the abundance of a limited number of mRNA and protein targets in the liver were observed, using batch RNA-Seq and proteomic screening; indicting the delivery of a miR-497a-5p mimic had functional consequences for the liver transcriptome and proteome. These data highlight the potential utility of commercially-available microRNA mimics in an in vivo setting, however, these data also highlight notable hurdles that still need to be overcome to facilitate the further use of microRNA mimics to study physiological processes in living, intact organisms.

This dataset

Open GSE270460 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE270460_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1126671 and SRA study SRP515350. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Samples in this study

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+ 18 more — browse all 18 samples with per-sample file links →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.