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Timing and location dictate monocyte fate and their transition to tumor-associated macrophages

GSE270793 Mus musculus Expression profiling by high throughput sequencing 9 samples Submitted 2024/08/02 Platform GPL24247
Summary
Tumor-associated macrophages (TAMs) are a heterogeneous population of cells whose phenotypes and functions are shaped by factors that are incompletely understood. Herein, we asked when and where TAMs arise from blood monocytes, and how they evolve during tumor development. We initiated pancreatic ductal adenocarcinoma (PDAC) in inducible monocyte fate-mapping mice and combined single-cell transcriptomics and high-dimensional flow cytometry to profile the monocyte-to-TAM transition. We revealed that monocytes differentiate first into a transient intermediate population of TAMs (IntTAM) that generates two longer-lived lineages of terminally differentiated TAMs with distinct gene expression profiles, phenotypes and intra-tumoral localization. Transcriptome datasets and tumor samples from patients with PDAC evidenced parallel TAM populations in humans and their prognostic associations. These insights will support the design of new therapeutic strategies targeting TAMs in PDAC.
Published in
Timing and location dictate monocyte fate and their transition to tumor-associated macrophages
Dunsmore G, Guo W, Li Z et al. · Science immunology 2024 · PMID 39058763 · doi:10.1126/sciimmunol.adk3981
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Also filed as BioProject PRJNA1128180 and SRA study SRP516104. Searching any of these in the dataset finder brings you back here.

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