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Overexpression of TSG101 causes cellular plasticity and development of adenosquamous mammary carcinoma

GSE271036 Mus musculus (mouse) Single-cell RNA-seq 13 samples Submitted 2025/07/16 Platform GPL24247Platform GPL19057
At a glance

Single-cell RNA-seq · mouse · 13 samples

What it is
Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart. Read from the summary: GEO's own type field only says 'expression profiling by sequencing', so confirm it in the paper.
Organism
Mus musculus (mouse)
Samples
13 samples: 5 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Overexpression of TSG101 causes the development of adenosquamous mammary carcinomaBreast cancer research : BCR · 2025
Summary (written by the submitters)
The mammalian Tumor Susceptibility Gene 101 (TSG101) encodes a protein with diverse functions that control the proliferation and survival of cells, but its role in malignant transformation has remained enigmatic. To study the pro-tumorigenic functions of TSG101, we developed a transgenic mouse model that expresses exogenous TSG101 along with a luciferase reporter in a ligand-controlled manner in the mammary gland epithelium.
Read the full summary ↓
Females overexpressing TSG101 develop ductal hyperplasia, adenomyoepitheliomas, and palpable adenosquamous carcinomas at an average latency of aproximately 10 months. These metaplastic mammary tumors are comprised of transforming basal and luminal epithelial cells, and using a GFP reporter strain to monitor the transgene activation on the single-cell level, we determined that the epithelial heterogeneity within transforming ducts and carcinomas was the result of cellular plasticity wihtin the luminal epithelium. On the molecular level, TSG101-induced mammary tumors are triple-negative and exhibit gene expression signatures of Wnt and inflammatory cytokine signaling, which are key regulators of epithelial cell fate. The ligand-controlled downregulation of exogenous TSG101 in established carcinomas led to tumor regression. We demonstrated that the TSG101-mediated activation of PI3K/AKT signaling as well as upregulation of Cyclin D1 and MDM2 are dependent on the perpetual expression of the oncoprotein. The collective findings of this study provide in vivo evidence that TSG101 has pro-tumorigenic properties that extend to cancer progression and maintenance, suggesting that this protein could be a rational molecular target to prevent and treat a subset of mammary tumors.
Published in
Overexpression of TSG101 causes the development of adenosquamous mammary carcinoma
Dennaoui R, Rädler PD, Wicker MN et al. · Breast cancer research : BCR 2025 · PMID 40624726 · doi:10.1186/s13058-025-02007-8
This dataset
Open GSE271036 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE271036_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA1129462 and SRA study SRP516800. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40624726) ↗
Study design
5 conditions, mostly in triplicate
Mammary tumor, MtTA Teto-TSG101wt, Mouse ×3 Mammary tumor, MtTA Teto-TSG101wt, Mouse 5138… ×3 Mammary Gland, WT FVB, Mouse ×3 Mammary tumor, MtTA Teto-TSG101wt, Mouse 5106… ×2 Mammary tumor, MtTA Teto-TSG101wt, Mouse 5135… ×2

Supports a case/control comparison: 10 samples read as cases, 3 as controls.

How we read this: 5 replicated groups read from 13 sample titles; they account for 13 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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