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Overcoming ABCB1 mediated multidrug resistance in castration resistant prostate cancer

GSE271075 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2024/08/08 Platform GPL24676
Summary
The few available therapies for mCRPC patients include taxanes docetaxel (DTX) and cabazitaxel (CBZ). However, development of resistance limits their clinical use. PCa that relapses after hormonal therapies, referred to as castration resistant prostate cancer (CRPC), often presents with metastases (mCRPC) that are the major cause of mortality. The few available therapies for mCRPC patients include taxanes docetaxel (DTX) and cabazitaxel (CBZ). However, development of resistance limits their clinical use. To study taxanes resistance, we produced CBZ resistant C4-2B cells (RC4-2B) and documented resistance to both CBZ and DTX in cell culture and in 3D prostaspheres settings. RNAseq identified increased expression of ABCB1 in RC4-2B, that was confirmed by immunoblotting and immunofluorescent analysis. ABCB1-specific inhibitor elacridar reversed CBZ and DTX resistance in RC4-2B cells, confirming ABCB1-mediated resistance mechanism.
Published in
Overcoming ABCB1 mediated multidrug resistance in castration resistant prostate cancer
Sarwar S, Morozov VM, Newcomb MA et al. · Cell death & disease 2024 · PMID 39090086 · doi:10.1038/s41419-024-06949-3
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Direct links to NCBI, no account and no request form: the whole study as GSE271075_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1129561 and SRA study SRP516849. Searching any of these in the dataset finder brings you back here.

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