Generation of self-renewing neuromesodermal progenitors with neuronal and skeletal muscle bipotential from human embryonic stem cells
Single-cell RNA-seq · human · 15 samples
- What it is
- Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart. Read from the summary: GEO's own type field only says 'expression profiling by sequencing', so confirm it in the paper.
- Organism
- Homo sapiens (human)
- Samples
- 15 samples: 13 distinct titles, 2 of them repeatedGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Generation of self-renewing neuromesodermal progenitors with neuronal and skeletal muscle bipotential from human embryonic stem cellsCell reports methods · 2024
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE271234_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.
Also filed as BioProject PRJNA1130495 and SRA study SRP517384. Searching any of these in the dataset finder brings you back here.
How we read this: Read from 15 sample titles: 13 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8371990 H1_Dorsal_neurons
- GSM8371991 H1_RABC_7D
- GSM8371992 H1_RASS_7D
- GSM8371993 H1_srNMPs_P25
- GSM8371994 H1_srNMPs_P8
- GSM8371995 H1_ventral_neurons
- GSM8371996 Hues9
- GSM8371997 Hues9_Dorsal_neurons
- GSM8371998 Hues9_RABC_7D
- GSM8371999 Hues9_RASS_7D
- GSM8372000 Hues9_muscle_40D
- GSM8372001 Hues9_muscle_50D
- GSM8372002 Hues9_srNMPs_P25
- GSM8372003 Hues9_srNMPs_P8
- GSM8372004 Hues9_ventral_neurons
- GSE266683 Patterning effects of FGF17 and cAMP on generation of dopaminergic progenitors for cell replacement therapy in Parkinson’s disease. [RNA-Seq] 24 samples shares progenitors, generation
- GSE243931 Generation of Human Expandable Progenitors with Limb-Bud Features via Chemically Induced Dedifferentiation 12 samples shares progenitors, generation
- GSE89057 RNA-seq of SOX5 overexpressing primary human neuronal progenitors 12 samples shares progenitors, neuronal
- GSE245723 Generation of expandable multipotential distal lung progenitors from human pluripotent stem cells that model idiopathic pulmonary fibrosis 9 samples shares progenitors, generation
- GSE294255 ALDH1A1+ cells are endometrial progenitors contributing to regeneration and endometriosis [Human] 8 samples shares progenitors, generation
- GSE266685 Patterning effects of FGF17 and cAMP on generation of dopaminergic progenitors for cell replacement therapy in Parkinson’s disease. [scRNA-Seq] 8 samples shares progenitors, generation
- GSE277159 Neuronal lineage tracing from progenitors in human cortical organoids reveals mechanisms of neuronal production, diversity, and disease 7 samples shares progenitors, neuronal
- GSE245721 Generation of expandable multipotential distal lung progenitors from human pluripotent stem cells that model idiopathic pulmonary fibrosis [bulk RNA-Seq] 6 samples shares progenitors, generation
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.