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Tracking changes in functionality and morphology of repopulated microglia in young and old mice

GSE271560 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2024/11/27 Platform GPL24247
Summary
Microglia (MG) are resident myeloid cells in the central nervous system (CNS) that perform homeostatic or pathological functions. Microglia self-renewal is controlled by the macrophage colony-stimulating factor-1 (CSF-1). Inhibitors of CSF-1 receptor (i.a. BLZ-945) by blocking the CSF1R signaling deplete 99% of microglia in 21 days and after cessation of treatment microglia rapidly repopulate and restore normal density within 1 week in adult mice. The functions of these repopulated microglia are poorly known and they can vary according to age. To investigate the microglia repopulation process, we compared samples of repopulated microglia from young (3 months) and old (12 months) mice using single cell RNA sequencing (scRNA-seq).
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Tracking changes in functionality and morphology of repopulated microglia in young and old mice
Luczak-Sobotkowska ZM, Rosa P, Lopez MB et al. · Journal of neuroinflammation 2024 · PMID 39363245 · doi:10.1186/s12974-024-03242-0
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Also filed as BioProject PRJNA1132223 and SRA study SRP523705. Searching any of these in the dataset finder brings you back here.

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