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Total RNA-seq and long-read RNAseq (Nanopore) upon Son KD in mouse embryonic stem cells

GSE271659 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2024/08/05 Platform GPL24973Platform GPL24247
Summary
Gene-expression noise can influence cell-fate choices across pathology and physiology. However, a crucial question persists: do regulatory proteins or pathways exist that control noise independently of mean expression levels? Resulting from a previous screen, the protein SON was identified as a potential noise regulator. We perform Son KD and utilize (1) total RNA sequencing (RNA-seq) to analyze differences in splicing efficiency of introns upon Son KD; and (2) Nanopore sequencing to analyze changes in isoform usage. These datasets correspond to the aforementioned total RNA-seq and long-read polyA+ cDNA Nanopore sequencing upon Son KD.
Published in
Multimodal screen identifies noise-regulatory proteins
García-Blay Ó, Hu X, Wassermann CL et al. · Developmental cell 2025 · PMID 39406240 · doi:10.1016/j.devcel.2024.09.015
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Also filed as BioProject PRJNA1132708 and SRA study SRP518367. Searching any of these in the dataset finder brings you back here.

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