GEO series

Evaluation of the Mammalian Aquaporin Inhibitors Auphen and Z433927330 in Treating Breast Cancer

GSE271926 Homo sapiens (human) RNA-seq (gene expression) 16 samples Submitted 2024/08/01 Platform GPL24676Platform GPL24247

At a glance

RNA-seq (gene expression) · human · 16 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Homo sapiens (human)
Topic: Breast cancerThis series is part of our curated briefing on it.
Samples: 16 samples: 4 × MCF-7, Vehicle, vs 4 × MCF-7, Auphen, vs 4 × PyMT, Vehicle, vs 4 × PyMT, Auphen,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Evaluation of the Mammalian Aquaporin Inhibitors Auphen and Z433927330 in Treating Breast CancerCancers · 2024

Summary (written by the submitters)

AQPs contribute to breast cancer progression and metastasis. We previously found that genetic inhibition of Aqp7 reduces primary tumor burden and metastasis in breast cancer. In this study, we utilized two AQP inhibitors, Auphen and Z433927330, to evaluate the efficacy of therapeutic inhibition of AQPs in breast cancer treatment. The inhibitors were evaluated in breast cancer for both cytotoxicity and in metabolic stability assays across both murine and human breast cancer cell lines.

Read the full summary ↓

Both AQP inhibitors also affected expression of other AQP transcripts and proteins, which demonstrates compensatory regulation between AQP family members. As a single agent, Auphen treatment in vivo extended overall survival but did not impact primary or metastatic tumor burden. However, Auphen treatment made cells more responsive to chemotherapy (doxorubicin) or endocrine treatment (tamoxifen, fulvestrant). In fact, treatment with Tamoxifen reduced the overall AQP7 protein expression. RNA-Seq of breast cancer cells treated with Auphen identified mitochondrial metabolism genes as impacted by Auphen and may contribute to reducing mammary tumor progression, lung metastasis, and increased therapeutic efficacy of endocrine therapy in breast cancer. Interestingly, we found that Auphen and tamoxifen cooperate to reduce breast cancer cell viability, which suggests that Auphen treatment makes the cells more susceptible to Tamoxifen. Together, this study highlights AQPs as therapeutic vulnerabilities of breast cancer metastasis that are promising and should be exploited. However, the pharmacologic results suggest additional chemical refinements and optimization of AQP inhibition are needed to make these AQP inhibitors appropriate to use for therapeutic benefit in overcoming endocrine therapy resistance.

Published in

Evaluation of the Mammalian Aquaporin Inhibitors Auphen and Z433927330 in Treating Breast Cancer

Charlestin V, Tan E, Arias-Matus CE et al. · Cancers 2024 · PMID 39123442 · doi:10.3390/cancers16152714

This dataset

Open GSE271926 in finder → Find more RNA-seq datasets → Breast cancer landscape →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE271926_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1134137 and SRA study SRP519073. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 39123442) ↗

Study design

4 × MCF-7, Vehicle, vs 4 × MCF-7, Auphen, vs 4 × PyMT, Vehicle, vs 4 × PyMT, Auphen,

Supports a between-group comparison across 16 samples.

How we read this: 4 replicated groups read from 16 sample titles; they account for 16 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8389190 MCF-7, Vehicle, replicate 1 MCF-7, Vehicle,
GSM8389191 MCF-7, Vehicle, replicate 2 MCF-7, Vehicle,
GSM8389192 MCF-7, Vehicle, replicate 3 MCF-7, Vehicle,
GSM8389193 MCF-7, Vehicle, replicate 4 MCF-7, Vehicle,
GSM8389194 MCF-7, Auphen, replicate 1 MCF-7, Auphen,
GSM8389195 MCF-7, Auphen, replicate 2 MCF-7, Auphen,
GSM8389196 MCF-7, Auphen, replicate 3 MCF-7, Auphen,
GSM8389197 MCF-7, Auphen, replicate 4 MCF-7, Auphen,
GSM8389198 PyMT, Vehicle, replicate 1 PyMT, Vehicle,
GSM8389199 PyMT, Vehicle, replicate 2 PyMT, Vehicle,
GSM8389200 PyMT, Vehicle, replicate 3 PyMT, Vehicle,
GSM8389201 PyMT, Vehicle, replicate 4 PyMT, Vehicle,
GSM8389202 PyMT, Auphen, replicate 1 PyMT, Auphen,
GSM8389203 PyMT, Auphen, replicate 2 PyMT, Auphen,
GSM8389204 PyMT, Auphen, replicate 3 PyMT, Auphen,
GSM8389205 PyMT, Auphen, replicate 4 PyMT, Auphen,

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.