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AMPK phosphosite profiling by label-free mass spectrometry reveals a multitude of mTORC1-regulated substrates

GSE272077 Homo sapiens Expression profiling by high throughput sequencing 8 samples Submitted 2025/02/27 Platform GPL24676
Summary
The metabolic kinase AMPK regulates transcription in response to nutrient status. AMPK is highly phosphorylated at position Ser184 in the regulatory beta2-subunit, however it's function is unknown. Our preliminary data indicate AMPK dephosphorylated at beta2-Ser184 has elevated nuclear activity and down-regulates apoptotic pathways in response to environmental insult. Here we compare transcriptomes of HEK293T cells expressing either endogenous level beta2 WT or the non-phosphorylatable mutant beta2-Ser182Ala, to gain insight into functional effects of elevated nuclear AMPK activity
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AMPK phosphosite profiling by label-free mass spectrometry reveals a multitude of mTORC1-regulated substrates
Smiles WJ, Ovens AJ, Yu D et al. · npj metabolic health and disease 2025 · PMID 40052110 · doi:10.1038/s44324-025-00052-7
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Also filed as BioProject PRJNA1134815 and SRA study SRP519434. Searching any of these in the dataset finder brings you back here.

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