NDF-FACT phase separation condensates are required for RNA Polymerase II transcription elongation through chromatin [RNA-Seq]
RNA-seq (gene expression) · human · 12 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 12 samples: 6 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Phase-separated NDF-FACT condensates facilitate transcription elongation on chromatinNature cell biology · 2025
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE273681_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1142604 and SRA study SRP523444. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 12 samples.
How we read this: 6 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8435071 iPSC, NDF-WT, RNA_seq_Rep1 iPSC, NDF-WT, RNA_seq
- GSM8435072 iPSC, NDF-K, RNA_seq_Rep1 iPSC, NDF-K, RNA_seq
- GSM8435073 iPSC, NDF-WT, RNA_seq_Rep2 iPSC, NDF-WT, RNA_seq
- GSM8435074 iPSC, NDF-K, RNA_seq_Rep2 iPSC, NDF-K, RNA_seq
- GSM9069731 CPC, NDF-WT, RNA_seq_Rep1 CPC, NDF-WT, RNA_seq
- GSM9069732 CPC, NDF-WT, RNA_seq_Rep2 CPC, NDF-WT, RNA_seq
- GSM9069733 CPC, NDF-K, RNA_seq_Rep1 CPC, NDF-K, RNA_seq
- GSM9069734 CPC, NDF-K, RNA_seq_Rep2 CPC, NDF-K, RNA_seq
- GSM9069735 EpiC-FB, NDF-WT, RNA_seq_Rep1 EpiC-FB, NDF-WT, RNA_seq
- GSM9069736 EpiC-FB, NDF-WT, RNA_seq_Rep2 EpiC-FB, NDF-WT, RNA_seq
- GSM9069737 EpiC-FB, NDF-K, RNA_seq_Rep1 EpiC-FB, NDF-K, RNA_seq
- GSM9069738 EpiC-FB, NDF-K, RNA_seq_Rep2 EpiC-FB, NDF-K, RNA_seq
- GSE301505 Mutual Antagonism Between PRC1 Condensates and SWI/SNF in Chromatin Regulation 210 samples shares condensates, chromatin
- GSE196619 Synthetic transcription factor overrides persistent repressive chromatin marks 120 samples shares transcription, chromatin
- GSE296192 Hypoxic regulation of chromatin and gene transcription 98 samples shares transcription, chromatin
- GSE296190 Hypoxic regulation of chromatin and gene transcription [ChIP-seq] 84 samples shares transcription, chromatin
- GSE182605 Integrative gene regulatory analysis reveals transcriptional mechanisms required in mature human hepatocytes and during in vitro hepatocyte differentiation 35 samples shares transcription, required
- GSE171817 SUMOylation Regulates Super-Enhancers Through Transcription Factor TFAP2C Binding on Chromatin 30 samples shares transcription, chromatin
- GSE300575 Cohesin acts as a transcriptional gatekeeper by restraining pause–release to promote processive elongation [ChIP-seq] 22 samples shares transcription, elongation
- GSE314310 Cardiopedia-Ligand: a Ligand Stimulation Atlas for Cardiac Function and Transcriptional Response 468 samples shares transcription
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.