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Non-catalytic role of SETD1A promotes gastric cancer cell proliferation through the E2F4-TAF6 axis in the cell cycle (RNA-seq)

GSE273825 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2025/07/25 Platform GPL24676Platform GPL18573
Summary
SETD1A catalyzes methylation of histone 3 lysine 4 (H3K4) and plays a critical role in development of multiple cancers. The abrrent expression of SETD1A relates to poor prognosis of gastric cancer (GC) patients. Here, we revealed that SETD1A is required for GC cell proliferation. Rescue experiment showed catalytic function is not necessary for GC cell growth and gene expression, whereas a novel non-catalytic FLOS domain is indispensable. The CRISPR screening targeting SETD1A targets identified that TAF6 acts as a downstream target of SETD1A which is essential for GC cell survival. Both SETD1A and TAF6 are required for G1/S cell cycle progression in GC cells. Non-catalytic component of SETD1A regulating expression of TAF6 through coactivating with E2F4s. These results demonstrate that non-canonical roles of SETD1A in GC cell, which supplys a potential therapeutic opportunity for GC.
Published in
Non-catalytic role of SETD1A promotes gastric cancer cell proliferation through the E2F4-TAF6 axis in the cell cycle
Ning M, Hoshii T, Nakagawa T et al. · Cell death & disease 2025 · PMID 40846851 · doi:10.1038/s41419-025-07976-4
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Also filed as BioProject PRJNA1143422 and SRA study SRP523871. Searching any of these in the dataset finder brings you back here.

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