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OpenTn5: Open-Source Resource for Robust and Scalable Tn5 Transposase Purification and Characterization [CUT&Tag]

GSE273909 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 8 samples Submitted 2025/09/02 Platform GPL30882
Summary
Tagmentation is an efficient approach for sequencing library preparation, which combines DNA fragmentation by transposition of sequencing adapters, using the bacterial cut-and-paste transposase Tn5. Here we present an open-source protocol for the generation of multi-purpose hyperactive Tn5 transposase, including its benchmarking in CUT&Tag, bulk and single-cell ATAC-seq. The OpenTn5 protocol yields multi-milligram quantities of pG-Tn5E54K, L372P protein per liter of E. coli culture, sufficient for thousands of tagmentation reactions and the enzyme retains activity in storage for more than a year.
Published in
OpenTn5: Open-Source Resource for Robust and Scalable Tn5 Transposase Purification and Characterization
Soroczynski J, Anderson LJ, Yeung JL et al. · bioRxiv : the preprint server for biology 2024 · PMID 39026714 · doi:10.1101/2024.07.11.602973
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Also filed as BioProject PRJNA1143887 and SRA study SRP524038. Searching any of these in the dataset finder brings you back here.

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