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Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos [ChIP-seq]

GSE273920 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 7 samples Submitted 2025/06/25 Platform GPL24247
Summary
Although specific transcription factors (TFs) are known to regulate cell fate decisions, the degree to which they can stimulate formation of specific cell organelles is less clear. We used a multiomics comparison of the transcriptomes of ciliated and unciliated embryonic cells to identify TFs up-regulated in ciliated cells. We also used conditional genetics in mouse embryos and stem cells and found that the TFs SP5 and SP8 regulate cilia formation and gene expression. In embryos lacking Sp5 and Sp8, primary and motile cilia were shorter than normal and reduced in number across cell types, contributing to situs inversus and hydrocephalus. Moreover, expression of SP8 was sufficient to induce primary cilia in unciliated cells. This work will facilitate the study of cilia assembly using stem cell models and promote further understanding of human ciliopathies.
Published in
Transcription factors SP5 and SP8 drive primary cilia formation
Liang Y, Koche R, Chalamalasetty RB et al. · bioRxiv : the preprint server for biology 2025 · PMID 40501818 · doi:10.1101/2025.06.03.657415
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Direct links to NCBI, no account and no request form: the whole study as GSE273920_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 7 samples. Raw sequencing reads are also available from ENA.

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Also filed as BioProject PRJNA1144105 and SRA study SRP524103. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40501818) ↗
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