Tripotent Lgr5 stem cells in the posterior tongue generate non-taste lingual, taste and salivary gland cells [Bulk RNA-seq]
Single-cell RNA-seq · mouse · 12 samples
- What it is
- Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart. Read from the summary: GEO's own type field only says 'expression profiling by sequencing', so confirm it in the paper.
- Organism
- Mus musculus (mouse)
- Samples
- 12 samples: 6 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Tripotent Lgr5 stem cells in the posterior tongue generate lingual, taste, and salivary gland lineagesNature communications · 2025
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE274014_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.
Also filed as BioProject PRJNA1144470 and SRA study SRP524272. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 12 samples.
How we read this: 6 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8441815 c2.9_1 c2.9
- GSM8441816 c2.9_2 c2.9
- GSM8441817 c2.10_1 c2.10
- GSM8441818 c2.10_2 c2.10
- GSM8441819 CVP_WT_1 CVP_WT
- GSM8441820 CVP_WT_2 CVP_WT
- GSM8441821 f2.4_1 f2.4
- GSM8441822 f2.4_2 f2.4
- GSM8441823 f2.5_1 f2.5
- GSM8441824 f2.5_2 f2.5
- GSM8441825 FoP_WT_1 FoP_WT
- GSM8441826 FoP_WT_2 FoP_WT
- GSE274015 Tripotent Lgr5 stem cells in the posterior tongue generate non-taste lingual, taste and salivary gland cells [scRNA-seq] 5 samples shares lgr5, tripotent, posterior, non-taste, generate, salivary, lingual, rna-seq
- GSE303692 A plastic EMP1⁺ to LGR5⁺ cell state conversion as a therapeutic bypass to KRAS-G12D inhibition in metastatic colorectal cancer [RNA-Seq] 24 samples shares lgr5, rna-seq
- GSE92865 Single-cell RNA-seq of Lgr5-eGFP+ intestinal stem cells upon Wnt and R-spondin modulation in vivo 14 samples shares lgr5, rna-seq
- GSE294825 A plastic EMP1⁺ to LGR5⁺ cell state conversion as a therapeutic bypass to KRAS-G12D inhibition in metastatic colorectal cancer [RNA-Seq mouse] 12 samples shares lgr5, rna-seq
- GSE92377 bulk cell RNA-seq of Lgr5+ ISCs with in vivo Rspo GOF and LOF 10 samples shares lgr5, rna-seq
- GSE333456 RNA-seq of Lgr5-GFP+ ISCs from KANSL1-deficient mice and control mice. 9 samples shares lgr5, rna-seq
- GSE202483 Analysis by RNA-seq of the transcriptional profile of three sub-populations of mouse colon epithelial cells (Lgr5+ columnar basal cells, goblet cells, enterocytes). 9 samples shares lgr5, rna-seq
- GSE272184 Lgr5 Marks Stem/Progenitor Cells Contributing to Epithelial and Muscle Development in the Mouse Esophagus [scRNA-Seq] 3 samples shares lgr5, rna-seq
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.